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Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury.

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  • Additional Information
    • Author-Supplied Keywords:
      neural stem cells
      neurotrophin-3
      regener
      Schwann cells
      spinal cord injury
      transplantation
    • NAICS/Industry Codes:
      541712 Research and Development in the Physical, Engineering, and Life Sciences (except Biotechnology)
    • Abstract:
      Study design:An animal model of transected spinal cord injury (SCI) was used to test the hypothesis that cografted neural stem cells (NSCs) and NT-3-SCs promote morphologic and functional recoveries of injured spinal cord.Objective:To explore whether cotransplant of NSCs and NT-3-SCs could promote the injured spinal cord repair.Setting:Zhongshan Medical College, Sun Yat-sen University, PR China.Methods:Female Sprague–Dawley (SD) rats weighing on 200–220 g were used to prepare SCI models. The spinal cord was transected between T9 and T10, then NSCs, SCs+NSCs, LacZ-SCs+NSCs, or NT-3-SCs+NSCs were grafted into the transected site.Results:(1) Part of NSCs could differentiate to neuron-like cells in the transected site and the percentage of differentiation was NT-3-SCs+NSCs group>SCs+NSCs group>NSCs group. (2) In the grafted groups, there were 5-HT, CGRP, and SP positive nerve fibres within the transected site. Some fluorogold (FG)-labeled cells were found in the spinal cord rostral to the transected site, the red nuclei and the inner pyramidal layer of sensorimotor cortex. (3) The cells grafted could enhance the injured neurons survival in inner pyramidal layer of sensorimotor cortex, red nuclei of midbrain, and Clark's nuclei of spinal cord's L1 segment, could decrease the latency and increase the amplitude of cortical somatosensory evoked potential (CSEP) and cortical motor evoked potential (CMEP), and could promote partly structural and functional recovery of the SCI rats.Conclusion:These results demonstrate that cografted NT-3-SCs and NSCs is a potential therapy for SCI.Sponsorship:This research was supported by Chinese National Key Project for Basic Research (G1999054009), Chinese National Natural Science Foundation (30270700) and Social Developmental Foundation of Guangdong Province (2003C33808) to YS Zeng; Natural Science Foundation of Guangdong Province (04300468) and Medical Science Research Grant of Guangdong Province (A2004081) to JS Guo.Spinal Cord (2007) 45, 15–24. doi:10.1038/sj.sc.3101943; published online 13 June 2006 [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of Spinal Cord is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Division of Neuroscience, Department of Histology and Embryology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou, China
      2Department of Histology and Embryology, Southern Medical University, Guangzhou, China
      3Cancer Center of Sun Yat-sen University, Guangzhou, China
      4Department of Orthopaedics, 3rd Affiliated Hospital, Sun Yet-sen University, Guangzhou, China
    • ISSN:
      1362-4393
    • Accession Number:
      10.1038/sj.sc.3101943
    • Accession Number:
      23580238