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LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p.

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  • Additional Information
    • Publication Year:
      2020
    • Author-Supplied Keywords:
      Apoptosis
      Gastric carcinoma
      LncRNA HOTTIP
      MiR-615-3p
      Proliferation
    • Abstract:
      OBJECTIVE: To explore the role of long-noncoding ribonucleic acid HOXA transcript at the distal tip (lncRNA HOTTIP) in the proliferation and apoptosis of gastric carcinoma cells. MATERIALS AND METHODS: The expressions of lncRNA HOTTIP in gastric carcinoma cell lines MGC-803, HGC-27, SNU-1, and SGC- 7901 and normal gastric mucosa cell line RGM- 1 were detected by real-time fluorescence quantitative polymerase chain reaction (PCR) and compared. The effects of lncRNA HOTTIP on proliferation and apoptosis of gastric carcinoma cells were detected by cell counting kit-8 (CCK- 8), colony formation assay, and flow cytometry, respectively. StarBase v2.0 website was adopted to predict the relationship between lncRNA HOTTIP and target miRNAs. Dual-Luciferase reporter assay was performed to verify the sponge effect of lncRNA HOTTIP on miR-615-3p. CCK- 8 experiment was conducted to detect its effect on proliferation of gastric carcinoma cells after co-silencing lncRNA HOTTIP and miR-615-3p. RESULTS: LncRNA HOTTIP was highly expressed in gastric carcinoma cell lines MGC- 803, HGC-27, SNU-1, and SGC-7901 than in normal gastric mucosa cell line RGM-1. After knockdown of lncRNA HOTTIP, the proliferation function of gastric carcinoma cells was markedly weakened, and the proportion of apoptotic cells increased. LncRNA HOTTIP was able to adsorb miR-615-3p via a sponge effect. Notably, knockdown of miR-615-3p restored the effect of silenced lncRNA HOTTIP on the proliferation function of gastric carcinoma cells. CONCLUSIONS: LncRNA HOTTIP is highly expressed in gastric carcinoma cells. It affects cell proliferation and apoptosis in gastric carcinoma by adsorbing miR-615-3p via a sponge effect. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of European Review for Medical & Pharmacological Sciences is the property of Verduci Editore and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • ISSN:
      11283602
    • Accession Number:
      144311486
  • Citations
    • ABNT:
      XIAO, Z.-S. et al. LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p. European Review for Medical & Pharmacological Sciences, [s. l.], v. 24, n. 12, p. 6692–6698, 2020. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edo&AN=144311486. Acesso em: 28 set. 2020.
    • AMA:
      XIAO Z-S, LONG H, ZHAO L, LI H-X, ZHANG X-N. LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p. European Review for Medical & Pharmacological Sciences. 2020;24(12):6692-6698. Accessed September 28, 2020. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edo&AN=144311486
    • APA:
      XIAO, Z.-S., LONG, H., ZHAO, L., LI, H.-X., & ZHANG, X.-N. (2020). LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p. European Review for Medical & Pharmacological Sciences, 24(12), 6692–6698.
    • Chicago/Turabian: Author-Date:
      XIAO, Z.-S., H. LONG, L. ZHAO, H.-X. LI, and X.-N. ZHANG. 2020. “LncRNA HOTTIP Promotes Proliferation and Inhibits Apoptosis of Gastric Carcinoma Cells via Adsorbing MiR-615-3p.” European Review for Medical & Pharmacological Sciences 24 (12): 6692–98. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edo&AN=144311486.
    • Harvard:
      XIAO, Z.-S. et al. (2020) ‘LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p’, European Review for Medical & Pharmacological Sciences, 24(12), pp. 6692–6698. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edo&AN=144311486 (Accessed: 28 September 2020).
    • Harvard: Australian:
      XIAO, Z-S, LONG, H, ZHAO, L, LI, H-X & ZHANG, X-N 2020, ‘LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p’, European Review for Medical & Pharmacological Sciences, vol. 24, no. 12, pp. 6692–6698, viewed 28 September 2020, .
    • MLA:
      XIAO, Z. S., et al. “LncRNA HOTTIP Promotes Proliferation and Inhibits Apoptosis of Gastric Carcinoma Cells via Adsorbing MiR-615-3p.” European Review for Medical & Pharmacological Sciences, vol. 24, no. 12, June 2020, pp. 6692–6698. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edo&AN=144311486.
    • Chicago/Turabian: Humanities:
      XIAO, Z.-S., H. LONG, L. ZHAO, H.-X. LI, and X.-N. ZHANG. “LncRNA HOTTIP Promotes Proliferation and Inhibits Apoptosis of Gastric Carcinoma Cells via Adsorbing MiR-615-3p.” European Review for Medical & Pharmacological Sciences 24, no. 12 (June 15, 2020): 6692–98. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edo&AN=144311486.
    • Vancouver/ICMJE:
      XIAO Z-S, LONG H, ZHAO L, LI H-X, ZHANG X-N. LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p. European Review for Medical & Pharmacological Sciences [Internet]. 2020 Jun 15 [cited 2020 Sep 28];24(12):6692–8. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edo&AN=144311486