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Chapter Nine - Immobilized Metal Affinity Chromatography (IMAC) for Metalloproteomics and Phosphoproteomics

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  • Author(s): Chang, Y.-Y.; Li, H.; Sun, H.
  • Source:
    In Inorganic and Organometallic Transition Metal Complexes with Biological Molecules and Living Cells 2017:329-353
  • Publication Information:
    Elsevier Inc.
  • Document Type:
    Book Chapter
  • Additional Information
    • Affiliation:
      The University of Hong Kong, Hong Kong, P.R. China
    • Keywords:
      IMAC
      Metallodrugs
      Metalloproteins
      Metalloproteome
      Metalloproteomics
      Phosphoproteomics
      Protein phosphorylation
    • Abstract:
      Although metalloproteins or metal-binding proteins have been ascertained to be associated with human health and diseases, the roles of metalloproteins in disease processes are not fully understood and require further comprehensive exploration. Immobilized metal affinity chromatography (IMAC) was initially developed for the purification of polyhistidine-tagged proteins, and subsequently for the analysis of phosphorylated proteins. Upon incorporation of different metal ions [e.g., Co(II), Cu(II)] onto solid resin, IMAC was further extended to track or enrich metal-binding proteins to elucidate their biological processes in various prokaryotic and eukaryotic cells. This chapter summarizes the progress of IMAC in metalloproteomics and phosphoproteomics, providing deeper insights into the mode of action of essential metal ions and metal-based drugs as well as the role of phosphorylated proteins in multiple cellular processes.
    • ISBN:
      978-0-12-803814-7
    • ISBN:
      978-0-12-803887-1
    • ISBN:
      0-12-803814-4
    • ISBN:
      0-12-803887-X
    • Accession Number:
      10.1016/B978-0-12-803814-7.00009-5
    • Accession Number:
      B9780128038147000095
    • Copyright:
      Copyright © 2017 Elsevier Inc. All rights reserved.
  • Citations
    • ABNT:
      CHANG, Y.-Y.; LI, H.; SUN, H. Chapter Nine - Immobilized Metal Affinity Chromatography (IMAC) for Metalloproteomics and Phosphoproteomics. Inorganic and Organometallic Transition Metal Complexes with Biological Molecules and Living Cells, [s. l.], p. 329–353, 2017. DOI 10.1016/B978-0-12-803814-7.00009-5. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edselp&AN=B9780128038147000095. Acesso em: 23 set. 2020.
    • AMA:
      Chang Y-Y, Li H, Sun H. Chapter Nine - Immobilized Metal Affinity Chromatography (IMAC) for Metalloproteomics and Phosphoproteomics. Inorganic and Organometallic Transition Metal Complexes with Biological Molecules and Living Cells. January 2017:329-353. doi:10.1016/B978-0-12-803814-7.00009-5
    • APA:
      Chang, Y.-Y., Li, H., & Sun, H. (2017). Chapter Nine - Immobilized Metal Affinity Chromatography (IMAC) for Metalloproteomics and Phosphoproteomics. Inorganic and Organometallic Transition Metal Complexes with Biological Molecules and Living Cells, 329–353. https://doi.org/10.1016/B978-0-12-803814-7.00009-5
    • Chicago/Turabian: Author-Date:
      Chang, Y.-Y., H. Li, and H. Sun. 2017. “Chapter Nine - Immobilized Metal Affinity Chromatography (IMAC) for Metalloproteomics and Phosphoproteomics.” Inorganic and Organometallic Transition Metal Complexes with Biological Molecules and Living Cells, January, 329–53. doi:10.1016/B978-0-12-803814-7.00009-5.
    • Harvard:
      Chang, Y.-Y., Li, H. and Sun, H. (2017) ‘Chapter Nine - Immobilized Metal Affinity Chromatography (IMAC) for Metalloproteomics and Phosphoproteomics’, Inorganic and Organometallic Transition Metal Complexes with Biological Molecules and Living Cells, pp. 329–353. doi: 10.1016/B978-0-12-803814-7.00009-5.
    • Harvard: Australian:
      Chang, Y-Y, Li, H & Sun, H 2017, ‘Chapter Nine - Immobilized Metal Affinity Chromatography (IMAC) for Metalloproteomics and Phosphoproteomics’, Inorganic and Organometallic Transition Metal Complexes with Biological Molecules and Living Cells, pp. 329–353, viewed 23 September 2020, .
    • MLA:
      Chang, Y. Y., et al. “Chapter Nine - Immobilized Metal Affinity Chromatography (IMAC) for Metalloproteomics and Phosphoproteomics.” Inorganic and Organometallic Transition Metal Complexes with Biological Molecules and Living Cells, Jan. 2017, pp. 329–353. EBSCOhost, doi:10.1016/B978-0-12-803814-7.00009-5.
    • Chicago/Turabian: Humanities:
      Chang, Y.-Y., H. Li, and H. Sun. “Chapter Nine - Immobilized Metal Affinity Chromatography (IMAC) for Metalloproteomics and Phosphoproteomics.” Inorganic and Organometallic Transition Metal Complexes with Biological Molecules and Living Cells, January 1, 2017, 329–53. doi:10.1016/B978-0-12-803814-7.00009-5.
    • Vancouver/ICMJE:
      Chang Y-Y, Li H, Sun H. Chapter Nine - Immobilized Metal Affinity Chromatography (IMAC) for Metalloproteomics and Phosphoproteomics. Inorganic and Organometallic Transition Metal Complexes with Biological Molecules and Living Cells [Internet]. 2017 Jan 1 [cited 2020 Sep 23];329–53. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edselp&AN=B9780128038147000095