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Effects of prebiotics on immunologic indicators and intestinal microbiota structure in perioperative colorectal cancer patients

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  • Additional Information
    • Affiliation:
      a Department of Colorectal Surgery, General Hospital of Ningxia Medical University, Yinchuan, China
      b College of Clinical Medicine, Ningxia Medical University, Yinchuan, China
      c Surgical Department, General Hospital of Ningxia Medical University, Yinchuan, China
      d Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan, China
      e Biobank of the General Hospital of Ningxia Medical University, Yinchuan, China
      f Nutrition Department, General Hospital of Ningxia Medical University, Yinchuan, China
      g Department of Gastroenterology, General Hospital of Ningxia Medical University, Yinchuan, China
    • Note:
      This study was supported by the Research and Development Plan of the 13th Five-Year Plan of the Ningxia autonomous region (major S&T projects; Grant No. 2016BZ02), First-Class Discipline Construction Founded Project of NingXia Medical University and the School of Clinical Medicine (Grant No. NXYLXK2017A05), Scientific Research Project grant funded by Ningxia High School (NGY2018-76), and Ningxia Medical University scientific research project XY201719. The authors also gratefully acknowledge funding by the National Natural Science Foundation of China (Grant No. 81460152). The authors thank the Shanghai Itechgene Technology Co. Ltd. for their support.
    • Keywords:
      Prebiotics
      Immune system
      Colorectal cancer
      Intestinal microbiota
      Operation
    • Abstract:
      Highlights •Prebiotic intake improved and changed immunologic indicators and intestinal microbiota structure in colorectal patients in the perioperative period.•Prebiotics increased the abundance of some commensal microbiota containing opportunistic pathogens in CRC patients.•Surgical stress decreased the abundance of most intestinal microbiota in the intestinal tract but increased the abundance of some opportunistic pathogens and commensals microbiota.•Bacteroides is a relevant bacteria for further research on the mechanism with prebiotics.
    • Abstract:
      Methods A randomized, double-blind, no-treatment parallel control clinical trial involving 140 perioperative patients (90 men and 50 women, aged 40–75 y) with CRC was performed. Patients were randomly divided into two groups: an intervention group (prebiotic group, n = 70) that received prebiotic supplementation of 30 g/d for 7 d, and a control group (non-prebiotic group, n = 70) that received no prebiotic supplementation. The nutritional and immunologic indices were evaluated for both groups before and after operation and analyzed against baseline values. Moreover, fecal samples were collected from 40 patients randomly chosen from the two groups to study intestinal microbiota, which was analyzed by sequencing the V3–V4 region of 16S ribosomal DNA using the Illumina (San Diego, CA) MiSeq (PE 2 × 300 bp) platform.
    • Abstract:
      Results Oral intake of prebiotics produced significant effects on immunologic indices in both the preoperative and postoperative periods, but the patterns of effects were different. In the preoperative period, prebiotics increased serum levels of immunoglobulin G (IgG; P = 0.02), IgM (P = 0.00), and transferrin (P = 0.027; all P < 0.05). In the postoperative period, enhanced levels of IgG (P = 0.003), IgA (P = 0.007), suppressor/cytotoxic T cells (CD3+CD8+; P = 0.043), and total B lymphocytes (CD19+; P = 0.012) were identified in the prebiotic group (all P < 0.05). The differences in the intestinal microbiota at the phylum level were not statistically significant between the intervention and control groups (P > 0.05). At the genus level, prebiotics increased the abundance of Bifidobacterium (P = 0.017) and Enterococcus (P = 0.02; both P < 0.05) but decreased the abundance of Bacteroides (P = 0.04) in the preoperative period (all P < 0.05). In the postoperative period, the abundance of Bacteroides (P = 0.04) was decreased, but the abundance of Enterococcus (P = 0.00), Bacillus (P = 0.01), Lactococcus (P = 0.00), and Streptococcus (P = 0.037) increased in the non-prebiotic group (all P < 0.05); however, no significant change was identified in the abundance of Enterococcus (P = 0.56), Lactococcus (P = 0.07), and Streptococcus (P = 0.56) as a result of prebiotic intervention in this period (all P > 0.05). The abundance of Escherichia-Shigella was increased after prebiotic intake in the postoperative period (P = 0.014, P < 0.05). There was a notable trend of decline in the abundance of intestinal microbiota from preoperative to postoperative in the non-prebiotic group.
    • Abstract:
      Conclusions Prebiotic intake is recommended to improve serum immunologic indicators in patients with CRC 7 d before operation. Prebiotics improved the abundance of four commensal microbiota containing opportunistic pathogens in patients with CRC. Surgical stress decreased the abundance of most intestinal microbiota in the intestinal tract but increased the abundance of some opportunistic pathogens and commensal microbiota. Bacteroides is a relevant bacterial species for further research on the mechanism of prebiotics.
    • ISSN:
      0899-9007
    • Accession Number:
      10.1016/j.nut.2018.10.038
    • Accession Number:
      S0899900718313200
    • Copyright:
      © 2018 The Authors. Published by Elsevier Inc.
  • Citations
    • ABNT:
      XIE, X. et al. Effects of prebiotics on immunologic indicators and intestinal microbiota structure in perioperative colorectal cancer patients. Nutrition, [s. l.], v. 61, p. 132–142, 2019. DOI 10.1016/j.nut.2018.10.038. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edselp&AN=S0899900718313200. Acesso em: 28 out. 2020.
    • AMA:
      Xie X, He Y, Li H, et al. Effects of prebiotics on immunologic indicators and intestinal microbiota structure in perioperative colorectal cancer patients. Nutrition. 2019;61:132-142. doi:10.1016/j.nut.2018.10.038
    • APA:
      Xie, X., He, Y., Li, H., Yu, D., Na, L., Sun, T., Zhang, D., Shi, X., Xia, Y., Jiang, T., Rong, S., Yang, S., Ma, X., & Xu, G. (2019). Effects of prebiotics on immunologic indicators and intestinal microbiota structure in perioperative colorectal cancer patients. Nutrition, 61, 132–142. https://doi.org/10.1016/j.nut.2018.10.038
    • Chicago/Turabian: Author-Date:
      Xie, Xiaoliang, Yaqin He, Hai Li, Dong Yu, Li Na, Ting Sun, Dong Zhang, et al. 2019. “Effects of Prebiotics on Immunologic Indicators and Intestinal Microbiota Structure in Perioperative Colorectal Cancer Patients.” Nutrition 61 (May): 132–42. doi:10.1016/j.nut.2018.10.038.
    • Harvard:
      Xie, X. et al. (2019) ‘Effects of prebiotics on immunologic indicators and intestinal microbiota structure in perioperative colorectal cancer patients’, Nutrition, 61, pp. 132–142. doi: 10.1016/j.nut.2018.10.038.
    • Harvard: Australian:
      Xie, X, He, Y, Li, H, Yu, D, Na, L, Sun, T, Zhang, D, Shi, X, Xia, Y, Jiang, T, Rong, S, Yang, S, Ma, X & Xu, G 2019, ‘Effects of prebiotics on immunologic indicators and intestinal microbiota structure in perioperative colorectal cancer patients’, Nutrition, vol. 61, pp. 132–142, viewed 28 October 2020, .
    • MLA:
      Xie, Xiaoliang, et al. “Effects of Prebiotics on Immunologic Indicators and Intestinal Microbiota Structure in Perioperative Colorectal Cancer Patients.” Nutrition, vol. 61, May 2019, pp. 132–142. EBSCOhost, doi:10.1016/j.nut.2018.10.038.
    • Chicago/Turabian: Humanities:
      Xie, Xiaoliang, Yaqin He, Hai Li, Dong Yu, Li Na, Ting Sun, Dong Zhang, et al. “Effects of Prebiotics on Immunologic Indicators and Intestinal Microbiota Structure in Perioperative Colorectal Cancer Patients.” Nutrition 61 (May 1, 2019): 132–42. doi:10.1016/j.nut.2018.10.038.
    • Vancouver/ICMJE:
      Xie X, He Y, Li H, Yu D, Na L, Sun T, et al. Effects of prebiotics on immunologic indicators and intestinal microbiota structure in perioperative colorectal cancer patients. Nutrition [Internet]. 2019 May 1 [cited 2020 Oct 28];61:132–42. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edselp&AN=S0899900718313200