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Uncoupling proteins 2 and 3 with age: regulation by fasting and beta3-adrenergic agonist treatment

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  • Source:
    Journals of Gerontology: Series A: Biological Sciences and Medical Sciences. Dec 2000, 55 (12). B588-B592. (5)
  • Subject Terms:
  • Additional Information
    • Author(s):
    • Abstract:
      In rodents, adaptive thermogenesis in brown adipose tissue (BAT) serves both to regulate body mass after hyperphagia and to conserve energy during food deprivation. In addition to uncoupling protein 1 (UCP1), UCP3 and possibly UCP2 may have a role in energy homeostasis in BAT. We examined basal levels of UCP2 and UCP3 mRNA with age and regulation of UCP1, UCP2, and UCP3 mRNA by two conditions known to modulate energy homeostasis: fasting and beta3-adrenergic agonists. UCP1, UCP2, and UCP3 mRNA levels were unchanged between 3, 24, and 31 months of age in BAT, and UCP2 and UCP3 mRNA levels were unchanged between 6 and 24 months of age in retroperitoneal white adipose tissue (RTWAT). Following a 2-day fast, there were sizable reductions in BAT UCP1 and UCP3 mRNA, but these decreases with fasting were significantly less in the older compared with the young rats. Fasting had no effect on UCP2 mRNA levels at any age. The beta3-adrenergic agonist, CL316,243, increased BAT UCP1 and UCP3 mRNA equally in both young and old rats. The beta3-adrenergic agonist did not increase UCP2 mRNA in BAT but did increase expression in RTWAT of both young and old rats. In summary, these data indicate that the expression of the three uncoupling proteins is unchanged with age. Although the upregulation of these uncoupling proteins by beta3-adrenergic agonist treatment is maintained with age, the downregulation by fasting is diminished with age. The parallel regulation of UCP1 and UCP3 expression in BAT suggests that UCP3, like UCP1, may have a role in energy homeostasis in BAT. The diminished downregulation of UCP1 and UCP3 expression in BAT by fasting suggests that energy conservation in response to food deprivation is impaired with age, and this may contribute to an inability of older animals to maintain body mass during periods when food is limited. ABSTRACT FROM AUTHOR (Copyright of Journals of Gerontology Series A: Biological Sciences & Medical Sciences is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.)
    • Accession Number:
      ADRENERGIC beta agonists; BROWN adipose tissue; RATS; PHYSIOLOGY
    • Publication Information:
      Publication Type: Journal Article ISSN: 1079-5006
    • Accession Number:
      EP4758915
  • Citations
    • ABNT:
      SCARPACE, P. J. et al. Uncoupling proteins 2 and 3 with age: regulation by fasting and beta3-adrenergic agonist treatment. Journals of Gerontology: Series A: Biological Sciences and Medical Sciences, [s. l.], v. 55, n. 12, p. B588–B592, 2000. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=gnh&AN=EP4758915. Acesso em: 19 set. 2020.
    • AMA:
      Scarpace PJ, Kumar MV, Hua Li, et al. Uncoupling proteins 2 and 3 with age: regulation by fasting and beta3-adrenergic agonist treatment. Journals of Gerontology: Series A: Biological Sciences and Medical Sciences. 2000;55(12):B588-B592. Accessed September 19, 2020. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=gnh&AN=EP4758915
    • APA:
      Scarpace, P. J., Kumar, M. V., Hua Li, Tumer, N., Scarpace, P. J., Kumar, M. V., Li, H., & Tümer, N. (2000). Uncoupling proteins 2 and 3 with age: regulation by fasting and beta3-adrenergic agonist treatment. Journals of Gerontology: Series A: Biological Sciences and Medical Sciences, 55(12), B588–B592.
    • Chicago/Turabian: Author-Date:
      Scarpace, Philip J., Monica V. Kumar, Hua Li, Nihal Tumer, P J Scarpace, M V Kumar, H Li, and N Tümer. 2000. “Uncoupling Proteins 2 and 3 with Age: Regulation by Fasting and Beta3-Adrenergic Agonist Treatment.” Journals of Gerontology: Series A: Biological Sciences and Medical Sciences 55 (12): B588–92. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=gnh&AN=EP4758915.
    • Harvard:
      Scarpace, Philip J. et al. (2000) ‘Uncoupling proteins 2 and 3 with age: regulation by fasting and beta3-adrenergic agonist treatment’, Journals of Gerontology: Series A: Biological Sciences and Medical Sciences, 55(12), pp. B588–B592. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=gnh&AN=EP4758915 (Accessed: 19 September 2020).
    • Harvard: Australian:
      Scarpace, Philip J., Kumar, Monica V., Hua Li, Tumer, N, Scarpace, P J, Kumar, M V, Li, H & Tümer, N 2000, ‘Uncoupling proteins 2 and 3 with age: regulation by fasting and beta3-adrenergic agonist treatment’, Journals of Gerontology: Series A: Biological Sciences and Medical Sciences, vol. 55, no. 12, pp. B588–B592, viewed 19 September 2020, .
    • MLA:
      Scarpace, Philip J., et al. “Uncoupling Proteins 2 and 3 with Age: Regulation by Fasting and Beta3-Adrenergic Agonist Treatment.” Journals of Gerontology: Series A: Biological Sciences and Medical Sciences, vol. 55, no. 12, Dec. 2000, pp. B588–B592. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=gnh&AN=EP4758915.
    • Chicago/Turabian: Humanities:
      Scarpace, Philip J., Monica V. Kumar, Hua Li, Nihal Tumer, P J Scarpace, M V Kumar, H Li, and N Tümer. “Uncoupling Proteins 2 and 3 with Age: Regulation by Fasting and Beta3-Adrenergic Agonist Treatment.” Journals of Gerontology: Series A: Biological Sciences and Medical Sciences 55, no. 12 (December 1, 2000): B588–92. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=gnh&AN=EP4758915.
    • Vancouver/ICMJE:
      Scarpace PJ, Kumar MV, Hua Li, Tumer N, Scarpace PJ, Kumar MV, et al. Uncoupling proteins 2 and 3 with age: regulation by fasting and beta3-adrenergic agonist treatment. Journals of Gerontology: Series A: Biological Sciences and Medical Sciences [Internet]. 2000 Dec 1 [cited 2020 Sep 19];55(12):B588–92. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=gnh&AN=EP4758915