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Antiproliferative effects of indole-3-acetamide/horseradish peroxidase in urinary organ cancer cells.

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  • Additional Information
    • Author(s):
    • Address:
      Department of Biochemistry, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Korea Republic.; [email protected]
    • Publication Information:
      Korea Republic
    • Abstract:
      Indole-3-acetamide (IAM) is an intermediate of the indole-3-acetic acid (IAA) biosynthetic pathways in plants. It is known that the combination of IAA and horseradish peroxidase (HRP) induces apoptosis in cancer cells. Here, we investigated effects of IAM/HRP treatment on PC-3 human prostate cancer cells and TCCSUP human urinary bladder carcinoma cells. First of all, we evaluated the effect of IAM on PC-3 and TCCSUP cell viability. IAM had no cytotoxic effects on PC-3 and TCCSUP cell viability. Next, we examined whether IAM/HRP could produce free radicals like IAA/HRP. DCF assay demonstrated that IAM/HRP produced free radicals in a dose-dependent manner, which is suppressed by Trolox, a water-soluble vitamin E derivative. Although mechanisms of IAM/HRP-induced free radical production remain unknown, we examined whether IAM/HRP could affect the proliferation of PC-3 and TCCSUP cells. MTT assay revealed that IAM/HRP decreased the proliferation of PC-3 and TCCSUP cells. Therefore, we suggest that IAM/HRP may provide a new potential candidate for urinary organ cancer treatment.
    • Number of References:
      13 ref.
    • Subject Terms:
      Public Health
    • Subject Terms:
    • Accession Number:
      anti-neoplastic properties, cancers
    • CABICODES:
      Non-communicable Human Diseases and Injuries (VV600)
    • Accession Number:
      60-35-5; 903-99-0
    • ISSN:
      0253-6250
    • Publication Information:
      Journal Article
    • Accession Number:
      20103040004
    • Copyright:
      ©2012 CAB International
  • Citations
    • ABNT:
      JEONG YUNMI et al. Antiproliferative effects of indole-3-acetamide/horseradish peroxidase in urinary organ cancer cells. Chung-Ang Journal of Medicine, [s. l.], v. 34, n. 3/4, p. 55–60, 2009. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20103040004. Acesso em: 22 out. 2020.
    • AMA:
      Jeong YunMi, Li HaiLan, Kim SuYeon, et al. Antiproliferative effects of indole-3-acetamide/horseradish peroxidase in urinary organ cancer cells. Chung-Ang Journal of Medicine. 2009;34(3/4):55-60. Accessed October 22, 2020. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20103040004
    • APA:
      Jeong YunMi, Li HaiLan, Kim SuYeon, Myung SoonChul, Lee MooYeol, Yun HyeYoung, Baek KwangJin, Kwon NyounSoo, & Kim DongSeok. (2009). Antiproliferative effects of indole-3-acetamide/horseradish peroxidase in urinary organ cancer cells. Chung-Ang Journal of Medicine, 34(3/4), 55–60.
    • Chicago/Turabian: Author-Date:
      Jeong YunMi, Li HaiLan, Kim SuYeon, Myung SoonChul, Lee MooYeol, Yun HyeYoung, Baek KwangJin, Kwon NyounSoo, and Kim DongSeok. 2009. “Antiproliferative Effects of Indole-3-Acetamide/Horseradish Peroxidase in Urinary Organ Cancer Cells.” Chung-Ang Journal of Medicine 34 (3/4): 55–60. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20103040004.
    • Harvard:
      Jeong YunMi et al. (2009) ‘Antiproliferative effects of indole-3-acetamide/horseradish peroxidase in urinary organ cancer cells’, Chung-Ang Journal of Medicine, 34(3/4), pp. 55–60. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20103040004 (Accessed: 22 October 2020).
    • Harvard: Australian:
      Jeong YunMi, Li HaiLan, Kim SuYeon, Myung SoonChul, Lee MooYeol, Yun HyeYoung, Baek KwangJin, Kwon NyounSoo & Kim DongSeok 2009, ‘Antiproliferative effects of indole-3-acetamide/horseradish peroxidase in urinary organ cancer cells’, Chung-Ang Journal of Medicine, vol. 34, no. 3/4, pp. 55–60, viewed 22 October 2020, .
    • MLA:
      Jeong YunMi, et al. “Antiproliferative Effects of Indole-3-Acetamide/Horseradish Peroxidase in Urinary Organ Cancer Cells.” Chung-Ang Journal of Medicine, vol. 34, no. 3/4, 2009, pp. 55–60. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20103040004.
    • Chicago/Turabian: Humanities:
      Jeong YunMi, Li HaiLan, Kim SuYeon, Myung SoonChul, Lee MooYeol, Yun HyeYoung, Baek KwangJin, Kwon NyounSoo, and Kim DongSeok. “Antiproliferative Effects of Indole-3-Acetamide/Horseradish Peroxidase in Urinary Organ Cancer Cells.” Chung-Ang Journal of Medicine 34, no. 3/4 (2009): 55–60. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20103040004.
    • Vancouver/ICMJE:
      Jeong YunMi, Li HaiLan, Kim SuYeon, Myung SoonChul, Lee MooYeol, Yun HyeYoung, et al. Antiproliferative effects of indole-3-acetamide/horseradish peroxidase in urinary organ cancer cells. Chung-Ang Journal of Medicine [Internet]. 2009 [cited 2020 Oct 22];34(3/4):55–60. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20103040004