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Evaluation of different immunoassays for the detection of anti-dsDNA-autoantibodies in patients with systemic lupus erythematosus (SLE).

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  • Additional Information
    • Author(s):
    • Publication Information:
      Germany
    • Abstract:
      The detection of anti-dsDNA antibodies plays an outstanding role in the diagnosis of systemic lupus erythematosus (SLE). However, the quality of methods used for SLE diagnosis may differ greatly. For this reason, we compared the diagnostic value of four commercial ELISAs (Farrzyme, Bindazyme, Orgentec and Varelisa) with that of the Fair radioimmunoassay (Farr-RIA) and the Crithidia luciliae immunofluorescence test (CLIF). Each was used to test serum samples from SLE patients versus control groups of patients with other inflammatory diseases. CLIF and Farr-RIA proved to be highly specific for SLE (99% and 88%, respectively). The diagnostic sensitivity of Farr-RIA was high (85%), whereas CLIF was significantly less sensitive (68%). The diagnostic specificity of the four ELISAs was lower (71% to 78%). In contrast, the diagnostic sensitivity of 3 out of 4 ELISAs was comparable or higher than that of Farr-RIA. The Farrzyme ELISA had slightly greater diagnostic specificity (78%), but was less sensitive (70%) than other ELISAs. Anti-dsDNA antibody detection by Farrzyme ELISA, Farr-RIA and CLIF was associated with renal involvement and low levels of complement components C3 and C4. Anti-dsDNA antibodies measured by these three assays and two ELISA methods (Bindazyme and Orgentec) were related to disease activity. These results confirm that there are notable quality differences between the studied anti-dsDNA antibody assays. CLIF and Farr-RIA are particularly valuable for SLE diagnosis. With CLIF and Farr-RIA, positive anti-dsDNA antibody results were obtained almost exclusively in SLE patients. Consequently, positive anti-dsDNA antibody results by the ELISA tests most commonly used in routine laboratories should be confirmed by CLIF and/or Farr-RIA to establish the definitive diagnosis of SLE. The ELISA methods are highly sensitive and can be used to monitor disease activity in patients with an already confirmed diagnosis of SLE.
    • Number of References:
      many ref.
    • Subject Terms:
      Public Health
    • Subject Terms:
    • Accession Number:
      antibody detection, antibody tests, dissertations, enzyme linked immunosorbent assay, fluorescent antibody technique, IFAT, immunoradiometric assay, radioimmunosorbent assay, serological diagnosis, serological techniques
    • CABICODES:
      Human Immunology and Allergology (VV055) (New March 2000)
      Non-communicable Human Diseases and Injuries (VV600)
      Diagnosis of Human Disease (VV720) (New March 2000)
      Techniques and Methodology (ZZ900)
    • Publication Information:
      Thesis; ISBN:9783863874971
    • Accession Number:
      20143306696
    • Copyright:
      ©2014 CAB International
  • Citations
    • ABNT:
      WASSIE, A. W. Evaluation of different immunoassays for the detection of anti-dsDNA-autoantibodies in patients with systemic lupus erythematosus (SLE). / Vergleichende Untersuchungen zur diagnostischen Relevanz von verschiedenen Immunoassay-Testsystemen zum Nachweis von Anti-dsDNA-Autoantikörpern beim Systemischen Lupus Erythematodes (SLE) des Menschen. 2014. Freie Universität Berlin, Berlin; Germany, 2014. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20143306696. Acesso em: 2 dez. 2020.
    • AMA:
      Wassie AW. Evaluation of different immunoassays for the detection of anti-dsDNA-autoantibodies in patients with systemic lupus erythematosus (SLE). / Vergleichende Untersuchungen zur diagnostischen Relevanz von verschiedenen Immunoassay-Testsystemen zum Nachweis von Anti-dsDNA-Autoantikörpern beim Systemischen Lupus Erythematodes (SLE) des Menschen. Vergleichende Untersuchungen zur diagnostischen Relevanz von verschiedenen Immunoassay-Testsystemen zum Nachweis von Anti-dsDNA-Autoantikörpern beim Systemischen Lupus Erythematodes (SLE) des Menschen. 2014:ii + 129. Accessed December 2, 2020. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20143306696
    • APA:
      Wassie, A. W. (2014). Evaluation of different immunoassays for the detection of anti-dsDNA-autoantibodies in patients with systemic lupus erythematosus (SLE). / Vergleichende Untersuchungen zur diagnostischen Relevanz von verschiedenen Immunoassay-Testsystemen zum Nachweis von Anti-dsDNA-Autoantikörpern beim Systemischen Lupus Erythematodes (SLE) des Menschen [Freie Universität Berlin]. In Vergleichende Untersuchungen zur diagnostischen Relevanz von verschiedenen Immunoassay-Testsystemen zum Nachweis von Anti-dsDNA-Autoantikörpern beim Systemischen Lupus Erythematodes (SLE) des Menschen (p. ii + 129).
    • Chicago/Turabian: Author-Date:
      Wassie, A. W. 2014. “Evaluation of Different Immunoassays for the Detection of Anti-DsDNA-Autoantibodies in Patients with Systemic Lupus Erythematosus (SLE). / Vergleichende Untersuchungen Zur Diagnostischen Relevanz von Verschiedenen Immunoassay-Testsystemen Zum Nachweis von Anti-DsDNA-Autoantikörpern Beim Systemischen Lupus Erythematodes (SLE) Des Menschen.” Vergleichende Untersuchungen Zur Diagnostischen Relevanz von Verschiedenen Immunoassay-Testsystemen Zum Nachweis von Anti-DsDNA-Autoantikörpern Beim Systemischen Lupus Erythematodes (SLE) Des Menschen. Berlin; Germany: Freie Universität Berlin. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20143306696.
    • Harvard:
      Wassie, A. W. (2014) Evaluation of different immunoassays for the detection of anti-dsDNA-autoantibodies in patients with systemic lupus erythematosus (SLE). / Vergleichende Untersuchungen zur diagnostischen Relevanz von verschiedenen Immunoassay-Testsystemen zum Nachweis von Anti-dsDNA-Autoantikörpern beim Systemischen Lupus Erythematodes (SLE) des Menschen, Vergleichende Untersuchungen zur diagnostischen Relevanz von verschiedenen Immunoassay-Testsystemen zum Nachweis von Anti-dsDNA-Autoantikörpern beim Systemischen Lupus Erythematodes (SLE) des Menschen. Freie Universität Berlin. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20143306696 (Accessed: 2 December 2020).
    • Harvard: Australian:
      Wassie, AW 2014, ‘Evaluation of different immunoassays for the detection of anti-dsDNA-autoantibodies in patients with systemic lupus erythematosus (SLE). / Vergleichende Untersuchungen zur diagnostischen Relevanz von verschiedenen Immunoassay-Testsystemen zum Nachweis von Anti-dsDNA-Autoantikörpern beim Systemischen Lupus Erythematodes (SLE) des Menschen’, Freie Universität Berlin, Berlin; Germany, Vergleichende Untersuchungen zur diagnostischen Relevanz von verschiedenen Immunoassay-Testsystemen zum Nachweis von Anti-dsDNA-Autoantikörpern beim Systemischen Lupus Erythematodes (SLE) des Menschen, p. ii + 129, viewed 2 December 2020, .
    • MLA:
      Wassie, A. W. “Evaluation of Different Immunoassays for the Detection of Anti-DsDNA-Autoantibodies in Patients with Systemic Lupus Erythematosus (SLE). / Vergleichende Untersuchungen Zur Diagnostischen Relevanz von Verschiedenen Immunoassay-Testsystemen Zum Nachweis von Anti-DsDNA-Autoantikörpern Beim Systemischen Lupus Erythematodes (SLE) Des Menschen.” Vergleichende Untersuchungen Zur Diagnostischen Relevanz von Verschiedenen Immunoassay-Testsystemen Zum Nachweis von Anti-DsDNA-Autoantikörpern Beim Systemischen Lupus Erythematodes (SLE) Des Menschen, Freie Universität Berlin, 2014, p. ii + 129. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20143306696.
    • Chicago/Turabian: Humanities:
      Wassie, A. W. “Evaluation of Different Immunoassays for the Detection of Anti-DsDNA-Autoantibodies in Patients with Systemic Lupus Erythematosus (SLE). / Vergleichende Untersuchungen Zur Diagnostischen Relevanz von Verschiedenen Immunoassay-Testsystemen Zum Nachweis von Anti-DsDNA-Autoantikörpern Beim Systemischen Lupus Erythematodes (SLE) Des Menschen.” Vergleichende Untersuchungen Zur Diagnostischen Relevanz von Verschiedenen Immunoassay-Testsystemen Zum Nachweis von Anti-DsDNA-Autoantikörpern Beim Systemischen Lupus Erythematodes (SLE) Des Menschen. Freie Universität Berlin, 2014. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20143306696.
    • Vancouver/ICMJE:
      Wassie AW. Evaluation of different immunoassays for the detection of anti-dsDNA-autoantibodies in patients with systemic lupus erythematosus (SLE). / Vergleichende Untersuchungen zur diagnostischen Relevanz von verschiedenen Immunoassay-Testsystemen zum Nachweis von Anti-dsDNA-Autoantikörpern beim Systemischen Lupus Erythematodes (SLE) des Menschen [Internet]. Vergleichende Untersuchungen zur diagnostischen Relevanz von verschiedenen Immunoassay-Testsystemen zum Nachweis von Anti-dsDNA-Autoantikörpern beim Systemischen Lupus Erythematodes (SLE) des Menschen. [Berlin; Germany]: Freie Universität Berlin; 2014 [cited 2020 Dec 2]. p. ii + 129. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20143306696