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Alternative testing strategies for predicting developmental toxicity of antifungal compounds.

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  • Additional Information
    • Author(s):
    • Publication Information:
      Netherlands
    • Abstract:
      This thesis aims to evaluate the applicability of integrated in vitro and in silico approach for prediction of developmental toxicity using a series of antifungal compounds (including fungicides and antifungal agents) as the model compounds. Chapter 2 presents the assessment of the usefulness of an in vitro BeWo cell transport model to predict placental transfer. Chapters 3 investigates the applicability of the ES-D3 cell differentiation assay combined with the in vitro BeWo transport model to predict relative in vivo developmental toxicity potencies. Chapter 4 extends the evaluation on this combined approach by testing another set of six antifungal compounds. Chapter 5 investigates the applicability of the ex ovo assay of chicken embryos to predict the potential to cause alterations in development of five antifungal compounds also studied in chapter 3 and investigates if the combination of the ex ovo assay with the kinetic information on placental transfer would improve its predictive capacity. In Chapter 6, the in vitro concentration-response curves of the antifungal compound tebuconazole, obtained in the ES-D3 cell differentiation assay (chapter 3) and the ex ovo assay of chicken embryos (chapter 5), are translated into in vivo dose-response curves for developmental toxicity of tebuconazole in rat, using the reverse dosimetry approach. Finally, Chapter 7 summarizes and discusses the results of the present thesis and provides future perspectives. It discusses possible implications of the use of the integrated in vitro-in silico approach for toxicological risk assessment and gives directions for future research in the field.
    • Number of References:
      96 ref.
    • Subject Terms:
      Plant Pathology;Poultry
    • Subject Terms:
    • Accession Number:
      adverse reactions, cytodifferentiation, fungistats
    • CABICODES:
      Pesticide and Drug Residues and Ecotoxicology (HH430) (New March 2000)
      Animal and in-vitro Models for Pharmaceuticals (VV450) (New March 2000)
      Mathematics and Statistics (ZZ100)
      Techniques and Methodology (ZZ900)
    • Accession Number:
      107534-96-3
    • Publication Information:
      Thesis; ISBN:9789462576780URL:http://edepot.wur.nl/375709
    • Accession Number:
      20163271351
    • Copyright:
      ©2016 CAB International
  • Citations
    • ABNT:
      LI HEQUN. Alternative testing strategies for predicting developmental toxicity of antifungal compounds. 2016. Wageningen University, Wageningen; Netherlands, 2016. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20163271351. Acesso em: 21 set. 2020.
    • AMA:
      Li HeQun. Alternative testing strategies for predicting developmental toxicity of antifungal compounds. Alternative testing strategies for predicting developmental toxicity of antifungal compounds. 2016:197. Accessed September 21, 2020. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20163271351
    • APA:
      Li HeQun. (2016). Alternative testing strategies for predicting developmental toxicity of antifungal compounds [Wageningen University]. In Alternative testing strategies for predicting developmental toxicity of antifungal compounds (p. 197).
    • Chicago/Turabian: Author-Date:
      Li HeQun. 2016. “Alternative Testing Strategies for Predicting Developmental Toxicity of Antifungal Compounds.” Alternative Testing Strategies for Predicting Developmental Toxicity of Antifungal Compounds. Wageningen; Netherlands: Wageningen University. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20163271351.
    • Harvard:
      Li HeQun (2016) Alternative testing strategies for predicting developmental toxicity of antifungal compounds, Alternative testing strategies for predicting developmental toxicity of antifungal compounds. Wageningen University. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20163271351 (Accessed: 21 September 2020).
    • Harvard: Australian:
      Li HeQun 2016, ‘Alternative testing strategies for predicting developmental toxicity of antifungal compounds’, Wageningen University, Wageningen; Netherlands, Alternative testing strategies for predicting developmental toxicity of antifungal compounds, p. 197, viewed 21 September 2020, .
    • MLA:
      Li HeQun. “Alternative Testing Strategies for Predicting Developmental Toxicity of Antifungal Compounds.” Alternative Testing Strategies for Predicting Developmental Toxicity of Antifungal Compounds, Wageningen University, 2016, p. 197. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20163271351.
    • Chicago/Turabian: Humanities:
      Li HeQun. “Alternative Testing Strategies for Predicting Developmental Toxicity of Antifungal Compounds.” Alternative Testing Strategies for Predicting Developmental Toxicity of Antifungal Compounds. Wageningen University, 2016. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20163271351.
    • Vancouver/ICMJE:
      Li HeQun. Alternative testing strategies for predicting developmental toxicity of antifungal compounds [Internet]. Alternative testing strategies for predicting developmental toxicity of antifungal compounds. [Wageningen; Netherlands]: Wageningen University; 2016 [cited 2020 Sep 21]. p. 197. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20163271351