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Dynamics of drug-resistant minor viral populations and phenotypic drug susceptibility in diverse HIV-1 subtypes.

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  • Additional Information
    • Author(s):
    • Address:
      Inst för laboratoriemedicin / Dept of Laboratory Medicine, Karolinska Institutet, Sweden.
    • Publication Information:
      Sweden
    • Abstract:
      The objective of the article was to drug resistance patterns of diverse HIV-1 subtypes at both genotypic and phenotypic level. A high throughput sequencing (HTS) method was utilized to amplify and sequence the gag-pol of different HIV-1 subtypes for identification and quantification of DRM present in <20% of the viral population. A novel bioinformatics pipeline was developed, MiDRMpol, which integrates genomic variations and mapping of minor populations with DRM. Phenotypic drug sensitivity assays were performed to study in vitro potency of newer ARV. Also, polymerase independent increase of virulence and replication competence of HIV-1, which may influence the outcome of ART, was studied. Identification and quantification of DRM at frequencies <20% is a major hurdle in current ART monitoring, and the MiDRMpol facilitates the analysis of such HTS data. However, it was also found that polymerase independent mutations which increase the replication capacity/virulence of HIV-1 may influence the outcome of ART. The new ARVs EFdA, CAB, and BIC suppress the viral load in vitro in a subtype independent manner. This is important since if not all subtypes are suppressed efficiently the risk of a global increase of acquired DRM would be further increased.
    • Number of References:
      many ref.
    • Subject Terms:
      Public Health
    • Subject Terms:
    • Accession Number:
      viral infections, antivirals
    • CABICODES:
      Genetics and Molecular Biology of Microorganisms (ZZ395) (New March 2000)
      Prion, Viral, Bacterial and Fungal Pathogens of Humans (VV210) (New March 2000)
      General Molecular Biology (ZZ360) (Discontinued March 2000)
      Pesticides and Drugs; Control (HH405) (New March 2000)
      Pesticide and Drug Resistance (HH410)
    • Publication Information:
      Thesis; URL:https://openarchive.ki.se/xmlui/handle/10616/46750
    • Accession Number:
      20203185651
    • Copyright:
      ©2019 CAB International
  • Citations
    • ABNT:
      GANESHAPPA ARALAGUPPE, S. P. Dynamics of drug-resistant minor viral populations and phenotypic drug susceptibility in diverse HIV-1 subtypes. 2019. Karolinska Institutet, Stockholm; Sweden, 2019. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20203185651. Acesso em: 10 ago. 2020.
    • AMA:
      Ganeshappa Aralaguppe SP. Dynamics of drug-resistant minor viral populations and phenotypic drug susceptibility in diverse HIV-1 subtypes. Dynamics of drug-resistant minor viral populations and phenotypic drug susceptibility in diverse HIV-1 subtypes. 2019:43. Accessed August 10, 2020. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20203185651
    • APA:
      Ganeshappa Aralaguppe, S. P. (2019). Dynamics of drug-resistant minor viral populations and phenotypic drug susceptibility in diverse HIV-1 subtypes [Karolinska Institutet]. In Dynamics of drug-resistant minor viral populations and phenotypic drug susceptibility in diverse HIV-1 subtypes (p. 43).
    • Chicago/Turabian: Author-Date:
      Ganeshappa Aralaguppe, S. P. 2019. “Dynamics of Drug-Resistant Minor Viral Populations and Phenotypic Drug Susceptibility in Diverse HIV-1 Subtypes.” Dynamics of Drug-Resistant Minor Viral Populations and Phenotypic Drug Susceptibility in Diverse HIV-1 Subtypes. Stockholm; Sweden: Karolinska Institutet. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20203185651.
    • Harvard:
      Ganeshappa Aralaguppe, S. P. (2019) Dynamics of drug-resistant minor viral populations and phenotypic drug susceptibility in diverse HIV-1 subtypes, Dynamics of drug-resistant minor viral populations and phenotypic drug susceptibility in diverse HIV-1 subtypes. Karolinska Institutet. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20203185651 (Accessed: 10 August 2020).
    • Harvard: Australian:
      Ganeshappa Aralaguppe, SP 2019, ‘Dynamics of drug-resistant minor viral populations and phenotypic drug susceptibility in diverse HIV-1 subtypes’, Karolinska Institutet, Stockholm; Sweden, Dynamics of drug-resistant minor viral populations and phenotypic drug susceptibility in diverse HIV-1 subtypes, p. 43, viewed 10 August 2020, .
    • MLA:
      Ganeshappa Aralaguppe, S. P. “Dynamics of Drug-Resistant Minor Viral Populations and Phenotypic Drug Susceptibility in Diverse HIV-1 Subtypes.” Dynamics of Drug-Resistant Minor Viral Populations and Phenotypic Drug Susceptibility in Diverse HIV-1 Subtypes, Karolinska Institutet, 2019, p. 43. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20203185651.
    • Chicago/Turabian: Humanities:
      Ganeshappa Aralaguppe, S. P. “Dynamics of Drug-Resistant Minor Viral Populations and Phenotypic Drug Susceptibility in Diverse HIV-1 Subtypes.” Dynamics of Drug-Resistant Minor Viral Populations and Phenotypic Drug Susceptibility in Diverse HIV-1 Subtypes. Karolinska Institutet, 2019. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20203185651.
    • Vancouver/ICMJE:
      Ganeshappa Aralaguppe SP. Dynamics of drug-resistant minor viral populations and phenotypic drug susceptibility in diverse HIV-1 subtypes [Internet]. Dynamics of drug-resistant minor viral populations and phenotypic drug susceptibility in diverse HIV-1 subtypes. [Stockholm; Sweden]: Karolinska Institutet; 2019 [cited 2020 Aug 10]. p. 43. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lhh&AN=20203185651