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Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling.

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  • Additional Information
    • Source:
      Publisher: American Diabetes Association Country of Publication: United States NLM ID: 0372763 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1939-327X (Electronic) Linking ISSN: 00121797 NLM ISO Abbreviation: Diabetes Subsets: Core Clinical (AIM); MEDLINE
    • Publication Information:
      Publication: Alexandria, VA : American Diabetes Association
      Original Publication: [New York, American Diabetes Association]
    • Subject Terms:
    • Abstract:
      Enteroendocrine cells (EECs) produce hormones such as glucagon-like peptide 1 and peptide YY that regulate food absorption, insulin secretion, and appetite. Based on the success of glucagon-like peptide 1-based therapies for type 2 diabetes and obesity, EECs are themselves the focus of drug discovery programs to enhance gut hormone secretion. The aim of this study was to identify the transcriptome and peptidome of human EECs and to provide a cross-species comparison between humans and mice. By RNA sequencing of human EECs purified by flow cytometry after cell fixation and staining, we present a first transcriptomic analysis of human EEC populations and demonstrate a strong correlation with murine counterparts. RNA sequencing was deep enough to enable identification of low-abundance transcripts such as G-protein-coupled receptors and ion channels, revealing expression in human EECs of G-protein-coupled receptors previously found to play roles in postprandial nutrient detection. With liquid chromatography-tandem mass spectrometry, we profiled the gradients of peptide hormones along the human and mouse gut, including their sequences and posttranslational modifications. The transcriptomic and peptidomic profiles of human and mouse EECs and cross-species comparison will be valuable tools for drug discovery programs and for understanding human metabolism and the endocrine impacts of bariatric surgery.
      (© 2019 by the American Diabetes Association.)
    • Comments:
      Comment in: Diabetes. 2019 May;68(5):904-905. (PMID: 31010883)
      Comment on: Diabetes. 2019 May;68(5):904-905. (PMID: 31010883)
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    • Grant Information:
      MC_UU_00014/5 United Kingdom MRC_ Medical Research Council; MC_UU_12012/5 United Kingdom MRC_ Medical Research Council; 106262 United Kingdom WT_ Wellcome Trust; United Kingdom WT_ Wellcome Trust; MC_UU_00014/3 United Kingdom MRC_ Medical Research Council; R01 DK100223 United States DK NIDDK NIH HHS; MC_UU_12012/3 United Kingdom MRC_ Medical Research Council; R01 DK110614 United States DK NIDDK NIH HHS; MR/M009041/1 United Kingdom MRC_ Medical Research Council; MR/M024873/1 United Kingdom MRC_ Medical Research Council
    • Accession Number:
      0 (Receptors, G-Protein-Coupled)
      89750-14-1 (Glucagon-Like Peptide 1)
    • Publication Date:
      Date Created: 20190209 Date Completed: 20190703 Latest Revision: 20200502
    • Publication Date:
      20200827
    • Accession Number:
      PMC6477899
    • Accession Number:
      10.2337/db18-0883
    • Accession Number:
      30733330
  • Citations
    • ABNT:
      ROBERTS, G. P. et al. Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling. Diabetes, [s. l.], v. 68, n. 5, p. 1062–1072, 2019. DOI 10.2337/db18-0883. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=30733330. Acesso em: 19 out. 2020.
    • AMA:
      Roberts GP, Larraufie P, Richards P, et al. Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling. Diabetes. 2019;68(5):1062-1072. doi:10.2337/db18-0883
    • APA:
      Roberts, G. P., Larraufie, P., Richards, P., Kay, R. G., Galvin, S. G., Miedzybrodzka, E. L., Leiter, A., Li, H. J., Glass, L. L., Ma, M. K. L., Lam, B., Yeo, G. S. H., Scharfmann, R., Chiarugi, D., Hardwick, R. H., Reimann, F., & Gribble, F. M. (2019). Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling. Diabetes, 68(5), 1062–1072. https://doi.org/10.2337/db18-0883
    • Chicago/Turabian: Author-Date:
      Roberts, Geoffrey P, Pierre Larraufie, Paul Richards, Richard G Kay, Sam G Galvin, Emily L Miedzybrodzka, Andrew Leiter, et al. 2019. “Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling.” Diabetes 68 (5): 1062–72. doi:10.2337/db18-0883.
    • Harvard:
      Roberts, G. P. et al. (2019) ‘Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling’, Diabetes, 68(5), pp. 1062–1072. doi: 10.2337/db18-0883.
    • Harvard: Australian:
      Roberts, GP, Larraufie, P, Richards, P, Kay, RG, Galvin, SG, Miedzybrodzka, EL, Leiter, A, Li, HJ, Glass, LL, Ma, MKL, Lam, B, Yeo, GSH, Scharfmann, R, Chiarugi, D, Hardwick, RH, Reimann, F & Gribble, FM 2019, ‘Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling’, Diabetes, vol. 68, no. 5, pp. 1062–1072, viewed 19 October 2020, .
    • MLA:
      Roberts, Geoffrey P., et al. “Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling.” Diabetes, vol. 68, no. 5, May 2019, pp. 1062–1072. EBSCOhost, doi:10.2337/db18-0883.
    • Chicago/Turabian: Humanities:
      Roberts, Geoffrey P, Pierre Larraufie, Paul Richards, Richard G Kay, Sam G Galvin, Emily L Miedzybrodzka, Andrew Leiter, et al. “Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling.” Diabetes 68, no. 5 (May 2019): 1062–72. doi:10.2337/db18-0883.
    • Vancouver/ICMJE:
      Roberts GP, Larraufie P, Richards P, Kay RG, Galvin SG, Miedzybrodzka EL, et al. Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling. Diabetes [Internet]. 2019 May [cited 2020 Oct 19];68(5):1062–72. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=30733330