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A microfluidic-induced C. elegans sleep state.

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  • Additional Information
    • Source:
      Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
    • Publication Information:
      Original Publication: [London] : Nature Pub. Group
    • Subject Terms:
    • Abstract:
      An important feature of animal behavior is the ability to switch rapidly between activity states, however, how the brain regulates these spontaneous transitions based on the animal's perceived environment is not well understood. Here we show a C. elegans sleep-like state on a scalable platform that enables simultaneous control of multiple environmental factors including temperature, mechanical stress, and food availability. This brief quiescent state, which we refer to as microfluidic-induced sleep, occurs spontaneously in microfluidic chambers, which allows us to track animal movement and perform whole-brain imaging. With these capabilities, we establish that microfluidic-induced sleep meets the behavioral requirements of C. elegans sleep and depends on multiple factors, such as satiety and temperature. Additionally, we show that C. elegans sleep can be induced through mechanosensory pathways. Together, these results establish a model system for studying how animals process multiple sensory pathways to regulate behavioral states.
    • Comments:
      Comment in: Lab Anim (NY). 2020 Jan;49(1):21. (PMID: 31819278)
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    • Grant Information:
      P40 OD010440 United States OD NIH HHS
    • Accession Number:
      0 (Sleep Aids, Pharmaceutical)
    • Publication Date:
      Date Created: 20191108 Date Completed: 20200302 Latest Revision: 20200309
    • Publication Date:
      20200716
    • Accession Number:
      PMC6834590
    • Accession Number:
      10.1038/s41467-019-13008-5
    • Accession Number:
      31695031
  • Citations
    • ABNT:
      GONZALES, D. L. et al. A microfluidic-induced C. elegans sleep state. Nature communications, [s. l.], v. 10, n. 1, p. 5035, 2019. DOI 10.1038/s41467-019-13008-5. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=31695031. Acesso em: 9 ago. 2020.
    • AMA:
      Gonzales DL, Zhou J, Fan B, Robinson JT. A microfluidic-induced C. elegans sleep state. Nature communications. 2019;10(1):5035. doi:10.1038/s41467-019-13008-5
    • APA:
      Gonzales, D. L., Zhou, J., Fan, B., & Robinson, J. T. (2019). A microfluidic-induced C. elegans sleep state. Nature Communications, 10(1), 5035. https://doi.org/10.1038/s41467-019-13008-5
    • Chicago/Turabian: Author-Date:
      Gonzales, Daniel L, Jasmine Zhou, Bo Fan, and Jacob T Robinson. 2019. “A Microfluidic-Induced C. Elegans Sleep State.” Nature Communications 10 (1): 5035. doi:10.1038/s41467-019-13008-5.
    • Harvard:
      Gonzales, D. L. et al. (2019) ‘A microfluidic-induced C. elegans sleep state’, Nature communications, 10(1), p. 5035. doi: 10.1038/s41467-019-13008-5.
    • Harvard: Australian:
      Gonzales, DL, Zhou, J, Fan, B & Robinson, JT 2019, ‘A microfluidic-induced C. elegans sleep state’, Nature communications, vol. 10, no. 1, p. 5035, viewed 9 August 2020, .
    • MLA:
      Gonzales, Daniel L., et al. “A Microfluidic-Induced C. Elegans Sleep State.” Nature Communications, vol. 10, no. 1, Nov. 2019, p. 5035. EBSCOhost, doi:10.1038/s41467-019-13008-5.
    • Chicago/Turabian: Humanities:
      Gonzales, Daniel L, Jasmine Zhou, Bo Fan, and Jacob T Robinson. “A Microfluidic-Induced C. Elegans Sleep State.” Nature Communications 10, no. 1 (November 6, 2019): 5035. doi:10.1038/s41467-019-13008-5.
    • Vancouver/ICMJE:
      Gonzales DL, Zhou J, Fan B, Robinson JT. A microfluidic-induced C. elegans sleep state. Nature communications [Internet]. 2019 Nov 6 [cited 2020 Aug 9];10(1):5035. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=31695031