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Polar or Charged Residues Located in Four Highly Conserved Motifs Play a Vital Role in the Function or pH Response of a UPF0118 Family Na + (Li + )/H + Antiporter.

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  • Additional Information
    • Source:
      Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE
    • Publication Information:
      Original Publication: Lausanne : Frontiers Research Foundation
    • Abstract:
      Functionally uncharacterized UPF0118 family has been re-designated as autoinducer-2 exporter (AI-2E) family since one of its members, Escherichia coli YdgG, was identified to function as an AI-2E. However, it's very likely that AI-2E family members may exhibit significantly distinct functions due to low identities between them. Recently, we identified one member of this family designated as UPF0118 to represent a novel class of Na + (Li + )/H + antiporters. In this study, we presented that UPF0118, together with its homologs, should represent an independent group of AI-2E family, designated as Na + /H + Antiporter Group. Notably, this group shows five highly conserved motifs designated as Motifs A to E, which are not detected in the majority of AI-2E family members. Functional analysis established that polar or charged residues located in Motif A to D play a vital role in Na + (Li + )/H + antiport activity or pH response of UPF0118. However, three basic residues located in Motif E are not involved in the function of UPF0118, although the truncation of C terminus resulted in the non-expression of this transporter. Therefore, we propose that E 179 -R 182 -K 215 -Q 217 -D 251 -R 292 -R 293 -E 296 -K 298 -S 30 7 located in Motifs A to D can be used for signature functional motifs to recognize whether AI-2E family members function as Na + (Li + )/H + antiporters. Current findings positively contribute to the knowledge of molecular mechanism of Na + , Li + transporting and pH response of UPF0118, and the functional prediction of uncharacterized AI-2E family members.
      (Copyright © 2020 Wang, Zou, Yan, Wang, Guo, Zhang, Song, Li, Chen, Shao, Meng and Jiang.)
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    • Contributed Indexing:
      Keywords: Na+(Li+) translocation; Na+(Li+)/H+ antiporter; UPF0118 family; functional motif; pH response
    • Publication Date:
      Date Created: 20200528 Latest Revision: 20200529
    • Publication Date:
      20200827
    • Accession Number:
      PMC7221264
    • Accession Number:
      10.3389/fmicb.2020.00841
    • Accession Number:
      32457721
  • Citations
    • ABNT:
      WANG, L. et al. Polar or Charged Residues Located in Four Highly Conserved Motifs Play a Vital Role in the Function or pH Response of a UPF0118 Family Na + (Li + )/H + Antiporter. Frontiers in microbiology, [s. l.], v. 11, p. 841, 2020. DOI 10.3389/fmicb.2020.00841. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=32457721. Acesso em: 1 out. 2020.
    • AMA:
      Wang L, Zou Q, Yan M, et al. Polar or Charged Residues Located in Four Highly Conserved Motifs Play a Vital Role in the Function or pH Response of a UPF0118 Family Na + (Li + )/H + Antiporter. Frontiers in microbiology. 2020;11:841. doi:10.3389/fmicb.2020.00841
    • APA:
      Wang, L., Zou, Q., Yan, M., Wang, Y., Guo, S., Zhang, R., Song, Y., Li, X., Chen, H., Shao, L., Meng, L., & Jiang, J. (2020). Polar or Charged Residues Located in Four Highly Conserved Motifs Play a Vital Role in the Function or pH Response of a UPF0118 Family Na + (Li + )/H + Antiporter. Frontiers in Microbiology, 11, 841. https://doi.org/10.3389/fmicb.2020.00841
    • Chicago/Turabian: Author-Date:
      Wang, Lidan, Qiao Zou, Mingxue Yan, Yuting Wang, Sijia Guo, Rui Zhang, Yang Song, et al. 2020. “Polar or Charged Residues Located in Four Highly Conserved Motifs Play a Vital Role in the Function or PH Response of a UPF0118 Family Na + (Li + )/H + Antiporter.” Frontiers in Microbiology 11 (May): 841. doi:10.3389/fmicb.2020.00841.
    • Harvard:
      Wang, L. et al. (2020) ‘Polar or Charged Residues Located in Four Highly Conserved Motifs Play a Vital Role in the Function or pH Response of a UPF0118 Family Na + (Li + )/H + Antiporter’, Frontiers in microbiology, 11, p. 841. doi: 10.3389/fmicb.2020.00841.
    • Harvard: Australian:
      Wang, L, Zou, Q, Yan, M, Wang, Y, Guo, S, Zhang, R, Song, Y, Li, X, Chen, H, Shao, L, Meng, L & Jiang, J 2020, ‘Polar or Charged Residues Located in Four Highly Conserved Motifs Play a Vital Role in the Function or pH Response of a UPF0118 Family Na + (Li + )/H + Antiporter’, Frontiers in microbiology, vol. 11, p. 841, viewed 1 October 2020, .
    • MLA:
      Wang, Lidan, et al. “Polar or Charged Residues Located in Four Highly Conserved Motifs Play a Vital Role in the Function or PH Response of a UPF0118 Family Na + (Li + )/H + Antiporter.” Frontiers in Microbiology, vol. 11, May 2020, p. 841. EBSCOhost, doi:10.3389/fmicb.2020.00841.
    • Chicago/Turabian: Humanities:
      Wang, Lidan, Qiao Zou, Mingxue Yan, Yuting Wang, Sijia Guo, Rui Zhang, Yang Song, et al. “Polar or Charged Residues Located in Four Highly Conserved Motifs Play a Vital Role in the Function or PH Response of a UPF0118 Family Na + (Li + )/H + Antiporter.” Frontiers in Microbiology 11 (May 7, 2020): 841. doi:10.3389/fmicb.2020.00841.
    • Vancouver/ICMJE:
      Wang L, Zou Q, Yan M, Wang Y, Guo S, Zhang R, et al. Polar or Charged Residues Located in Four Highly Conserved Motifs Play a Vital Role in the Function or pH Response of a UPF0118 Family Na + (Li + )/H + Antiporter. Frontiers in microbiology [Internet]. 2020 May 7 [cited 2020 Oct 1];11:841. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=32457721