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LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p.

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  • Author(s): Xiao ZS;Xiao ZS; Long H; Zhao L; Li HX; Zhang XN
  • Source:
    European review for medical and pharmacological sciences [Eur Rev Med Pharmacol Sci] 2020 Jun; Vol. 24 (12), pp. 6692-6698.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Verduci Country of Publication: Italy NLM ID: 9717360 Publication Model: Print Cited Medium: Internet ISSN: 2284-0729 (Electronic) Linking ISSN: 11283602 NLM ISO Abbreviation: Eur Rev Med Pharmacol Sci Subsets: In Process; MEDLINE
    • Publication Information:
      Original Publication: Rome : Verduci, [1997-
    • Abstract:
      Objective: To explore the role of long-noncoding ribonucleic acid HOXA transcript at the distal tip (lncRNA HOTTIP) in the proliferation and apoptosis of gastric carcinoma cells.
      Materials and Methods: The expressions of lncRNA HOTTIP in gastric carcinoma cell lines MGC-803, HGC-27, SNU-1, and SGC-7901 and normal gastric mucosa cell line RGM-1 were detected by real-time fluorescence quantitative polymerase chain reaction (PCR) and compared. The effects of lncRNA HOTTIP on proliferation and apoptosis of gastric carcinoma cells were detected by cell counting kit-8 (CCK-8), colony formation assay, and flow cytometry, respectively. StarBase v2.0 website was adopted to predict the relationship between lncRNA HOTTIP and target miRNAs. Dual-Luciferase reporter assay was performed to verify the sponge effect of lncRNA HOTTIP on miR-615-3p. CCK-8 experiment was conducted to detect its effect on proliferation of gastric carcinoma cells after co-silencing lncRNA HOTTIP and miR-615-3p.
      Results: LncRNA HOTTIP was highly expressed in gastric carcinoma cell lines MGC-803, HGC-27, SNU-1, and SGC-7901 than in normal gastric mucosa cell line RGM-1. After knockdown of lncRNA HOTTIP, the proliferation function of gastric carcinoma cells was markedly weakened, and the proportion of apoptotic cells increased. LncRNA HOTTIP was able to adsorb miR-615-3p via a sponge effect. Notably, knockdown of miR-615-3p restored the effect of silenced lncRNA HOTTIP on the proliferation function of gastric carcinoma cells.
      Conclusions: LncRNA HOTTIP is highly expressed in gastric carcinoma cells. It affects cell proliferation and apoptosis in gastric carcinoma by adsorbing miR-615-3p via a sponge effect.
    • Publication Date:
      Date Created: 20200708 Latest Revision: 20200707
    • Publication Date:
      20200827
    • Accession Number:
      10.26355/eurrev_202006_21656
    • Accession Number:
      32633359
  • Citations
    • ABNT:
      XIAO, Z.-S. et al. LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p. European review for medical and pharmacological sciences, [s. l.], v. 24, n. 12, p. 6692–6698, 2020. DOI 10.26355/eurrev_202006_21656. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=32633359. Acesso em: 20 out. 2020.
    • AMA:
      Xiao Z-S, Long H, Zhao L, Li H-X, Zhang X-N. LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p. European review for medical and pharmacological sciences. 2020;24(12):6692-6698. doi:10.26355/eurrev_202006_21656
    • APA:
      Xiao, Z.-S., Long, H., Zhao, L., Li, H.-X., & Zhang, X.-N. (2020). LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p. European Review for Medical and Pharmacological Sciences, 24(12), 6692–6698. https://doi.org/10.26355/eurrev_202006_21656
    • Chicago/Turabian: Author-Date:
      Xiao, Z-S, H Long, L Zhao, H-X Li, and X-N Zhang. 2020. “LncRNA HOTTIP Promotes Proliferation and Inhibits Apoptosis of Gastric Carcinoma Cells via Adsorbing MiR-615-3p.” European Review for Medical and Pharmacological Sciences 24 (12): 6692–98. doi:10.26355/eurrev_202006_21656.
    • Harvard:
      Xiao, Z.-S. et al. (2020) ‘LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p’, European review for medical and pharmacological sciences, 24(12), pp. 6692–6698. doi: 10.26355/eurrev_202006_21656.
    • Harvard: Australian:
      Xiao, Z-S, Long, H, Zhao, L, Li, H-X & Zhang, X-N 2020, ‘LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p’, European review for medical and pharmacological sciences, vol. 24, no. 12, pp. 6692–6698, viewed 20 October 2020, .
    • MLA:
      Xiao, Z. S., et al. “LncRNA HOTTIP Promotes Proliferation and Inhibits Apoptosis of Gastric Carcinoma Cells via Adsorbing MiR-615-3p.” European Review for Medical and Pharmacological Sciences, vol. 24, no. 12, June 2020, pp. 6692–6698. EBSCOhost, doi:10.26355/eurrev_202006_21656.
    • Chicago/Turabian: Humanities:
      Xiao, Z-S, H Long, L Zhao, H-X Li, and X-N Zhang. “LncRNA HOTTIP Promotes Proliferation and Inhibits Apoptosis of Gastric Carcinoma Cells via Adsorbing MiR-615-3p.” European Review for Medical and Pharmacological Sciences 24, no. 12 (June 2020): 6692–98. doi:10.26355/eurrev_202006_21656.
    • Vancouver/ICMJE:
      Xiao Z-S, Long H, Zhao L, Li H-X, Zhang X-N. LncRNA HOTTIP promotes proliferation and inhibits apoptosis of gastric carcinoma cells via adsorbing miR-615-3p. European review for medical and pharmacological sciences [Internet]. 2020 Jun [cited 2020 Oct 20];24(12):6692–8. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=32633359