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Protective effects of extract of Ginkgo biloba (EGb 761) on nerve cells after spinal cord injury in rats.

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  • Additional Information
    • Subject Terms:
    • Subject Terms:
    • Abstract:
      STUDY DESIGN: An experimental animal model was used to assess spinal cord injury following lateral hemitransection at thoracic spinal cord level. OBJECTIVE: To determine whether extract of Ginkgo biloba (EGb) could have a neuroprotective effect in spinal cord injury (SCI) in rats. SETTING: Department of Biological Sciences and Biotechnology, Tsinghua University, China. METHODS: A total of 72 adult rats were divided randomly into three groups: the EGb group, normal saline (NS) group, and sham operation group (sham group). After thoracic spinal cord hemitransection was performed at the level of the 9th thoracic vertebra (T9), rats in the EGb group were given 100 mg/kg EGb 761 daily, while rats in the NS group received NS. The rats in the sham group only underwent laminectomy without spinal cord hemitransection. At various time points after surgery, thoracic spinal cords were sampled and sliced for histochemistry, immunohistochemistry of inducible nitric oxide synthase (iNOS), and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) of apoptotic cells. RESULTS: Myelin staining showed that the area of cavities was small and the demyelinated zones were limited at and around the injury site of the spinal cord in the EGb group, while the area of cavities was large and the demyelinated zones were serious in the NS group. Nissl staining showed that the ratio of bilateral ventral horn neurons (transection side/uninjured side) in the EGb group was higher than that in the NS group (P<0.05). The apoptotic index and the percentage of iNOS-positive cells were lower in the EGb group than in the NS group. Furthermore, the percentage of iNOS-positive cells positively correlated with the apoptotic index (r( 2)=0.729, P<0.01) after SCI. CONCLUSION: This study demonstrated that EGb 761 could inhibit iNOS expression and have neuroprotective effect by preventing nerve cells from apoptosis after SCI in rats.
    • Journal Subset:
      Allied Health; Biomedical; Europe; Expert Peer Reviewed; Peer Reviewed; UK & Ireland
    • ISSN:
      1362-4393
    • MEDLINE Info:
      PMID: NLM16415923 NLM UID: 9609749
    • Publication Date:
      20080208
    • Publication Date:
      20150711
    • Accession Number:
      105961081
  • Citations
    • ABNT:
      AO Q et al. Protective effects of extract of Ginkgo biloba (EGb 761) on nerve cells after spinal cord injury in rats. Spinal Cord, [s. l.], v. 44, n. 11, p. 662–667, 2006. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=105961081. Acesso em: 22 out. 2020.
    • AMA:
      Ao Q, Sun XH, Wang AJ, et al. Protective effects of extract of Ginkgo biloba (EGb 761) on nerve cells after spinal cord injury in rats. Spinal Cord. 2006;44(11):662-667. Accessed October 22, 2020. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=105961081
    • APA:
      Ao Q, Sun XH, Wang AJ, Fu PF, Gong K, Zuo HC, Zuo HZ, Gong YD, & Zhang XF. (2006). Protective effects of extract of Ginkgo biloba (EGb 761) on nerve cells after spinal cord injury in rats. Spinal Cord, 44(11), 662–667.
    • Chicago/Turabian: Author-Date:
      Ao Q, Sun XH, Wang AJ, Fu PF, Gong K, Zuo HC, Zuo HZ, Gong YD, and Zhang XF. 2006. “Protective Effects of Extract of Ginkgo Biloba (EGb 761) on Nerve Cells after Spinal Cord Injury in Rats.” Spinal Cord 44 (11): 662–67. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=105961081.
    • Harvard:
      Ao Q et al. (2006) ‘Protective effects of extract of Ginkgo biloba (EGb 761) on nerve cells after spinal cord injury in rats’, Spinal Cord, 44(11), pp. 662–667. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=105961081 (Accessed: 22 October 2020).
    • Harvard: Australian:
      Ao Q, Sun XH, Wang AJ, Fu PF, Gong K, Zuo HC, Zuo HZ, Gong YD & Zhang XF 2006, ‘Protective effects of extract of Ginkgo biloba (EGb 761) on nerve cells after spinal cord injury in rats’, Spinal Cord, vol. 44, no. 11, pp. 662–667, viewed 22 October 2020, .
    • MLA:
      Ao Q, et al. “Protective Effects of Extract of Ginkgo Biloba (EGb 761) on Nerve Cells after Spinal Cord Injury in Rats.” Spinal Cord, vol. 44, no. 11, Nov. 2006, pp. 662–667. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=105961081.
    • Chicago/Turabian: Humanities:
      Ao Q, Sun XH, Wang AJ, Fu PF, Gong K, Zuo HC, Zuo HZ, Gong YD, and Zhang XF. “Protective Effects of Extract of Ginkgo Biloba (EGb 761) on Nerve Cells after Spinal Cord Injury in Rats.” Spinal Cord 44, no. 11 (November 2006): 662–67. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=105961081.
    • Vancouver/ICMJE:
      Ao Q, Sun XH, Wang AJ, Fu PF, Gong K, Zuo HC, et al. Protective effects of extract of Ginkgo biloba (EGb 761) on nerve cells after spinal cord injury in rats. Spinal Cord [Internet]. 2006 Nov [cited 2020 Oct 22];44(11):662–7. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=105961081