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MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma.

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  • Additional Information
    • Affiliation:
      Department of Pathology, Albert Einstein College of Medicine, Bronx, New York
      Department of Anatomy & Structural Biology, Albert Einstein College of Medicine, Bronx, New York
    • Series Title:
      Special Section&; 8212;Head and Neck Squamous Cell Carcinoma
    • Subject Terms:
    • Subject Terms:
    • Abstract:
      Context.--Head and neck squamous cell carcinoma (HNSCC) is a highly invasive cancer with an association with locoregional recurrence and lymph node metastasis. We have previously reported that low microRNA-375 (miR-375) expression levels correlate with poor patient survival, increased locoregional recurrence, and distant metastasis. Increasing miR-375 expression in HNSCC cell lines to levels found in normal cells results in suppressed invasive properties. HNSCC invasion is mediated in part by invadopodia-associated degradation of the extracellular matrix. Objective.--To determine whether elevated miR-375 expression in HNSCC cell lines also affects invadopodia formation and activity. Design.--For evaluation of the matrix degradation properties of the HNSCC lines, an invadopodial matrix degradation assay was used. The total protein levels of invadopodia-associated proteins were measured by Western blot analyses. Immunoprecipitation experiments were conducted to evaluate the tyrosine phosphorylation state of cortactin. Human protease arrays were used for the detection of the secreted proteases. Quantitative real time--polymerase chain reaction measurements were used to evaluate the messenger RNA (mRNA) expression of the commonly regulated proteases. Results.--Increased miR-375 expression in HNSCC cells suppresses extracellular matrix degradation and reduces the number of mature invadopodia. Higher miR-375 expression does not reduce cellular levels of selected invadopodia-associated proteins, nor is tyrosine phosphorylation of cortactin altered. However, HNSCC cells with higher miR-375 expression had significant reductions in the mRNA expression levels and secreted levels of specific proteases. Conclusions.--MicroRNA-375 regulates invadopodia maturation and function potentially by suppressing the expression and secretion of proteases.
    • Journal Subset:
      Allied Health; Biomedical; Peer Reviewed; USA
    • Special Interest:
      Laboratory Diagnosis; Oncologic Care
    • ISSN:
      0003-9985
    • MEDLINE Info:
      PMID: NLM26172508 NLM UID: 7607091
    • Grant Information:
      Supported by a predoctoral fellowship from National Institutes of Health (NIH) (CA168337)., a postdoctoral fellowship from Susan G. Komen for the Cure (KG111405), NIH grant (CA150344). NIH grants (CA77522 and CA100324) , and by theDepartment of Pathology, Albert Einstein College of Medicine/Montefiore Medical Center
    • Publication Date:
      20151109
    • Publication Date:
      20191111
    • DOI:
      10.5858/arpa.2014-0471-OA
    • Accession Number:
      110709109
  • Citations
    • ABNT:
      JIMENEZ, L. et al. MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma. Archives of Pathology & Laboratory Medicine, [s. l.], v. 139, n. 11, Special Section&; 8212;Head and Neck Squamous Cell Carcinoma, p. 1349–1361, 2015. DOI 10.5858/arpa.2014-0471-OA. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=110709109. Acesso em: 23 nov. 2020.
    • AMA:
      Jimenez L, Sharma VP, Condeelis J, et al. MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma. Archives of Pathology & Laboratory Medicine. 2015;139(11):1349-1361. doi:10.5858/arpa.2014-0471-OA
    • APA:
      Jimenez, L., Sharma, V. P., Condeelis, J., Harris, T., Ow, T. J., Prystowsky, M. B., Childs, G., & Segall, J. E. (2015). MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma. Archives of Pathology & Laboratory Medicine, 139(11), 1349–1361. https://doi.org/10.5858/arpa.2014-0471-OA
    • Chicago/Turabian: Author-Date:
      Jimenez, Lizandra, Ved P. Sharma, John Condeelis, Thomas Harris, Thomas J. Ow, Michael B. Prystowsky, Geoffrey Childs, and Jeffrey E. Segall. 2015. “MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma.” Archives of Pathology & Laboratory Medicine, Special Section&; 8212;Head and Neck Squamous Cell Carcinoma, 139 (11): 1349–61. doi:10.5858/arpa.2014-0471-OA.
    • Harvard:
      Jimenez, L. et al. (2015) ‘MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma’, Archives of Pathology & Laboratory Medicine. (Special Section&; 8212;Head and Neck Squamous Cell Carcinoma), 139(11), pp. 1349–1361. doi: 10.5858/arpa.2014-0471-OA.
    • Harvard: Australian:
      Jimenez, L, Sharma, VP, Condeelis, J, Harris, T, Ow, TJ, Prystowsky, MB, Childs, G & Segall, JE 2015, ‘MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma’, Archives of Pathology & Laboratory Medicine, vol. 139, no. 11, pp. 1349–1361, viewed 23 November 2020, .
    • MLA:
      Jimenez, Lizandra, et al. “MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma.” Archives of Pathology & Laboratory Medicine, vol. 139, no. 11, Nov. 2015, pp. 1349–1361. EBSCOhost, doi:10.5858/arpa.2014-0471-OA.
    • Chicago/Turabian: Humanities:
      Jimenez, Lizandra, Ved P. Sharma, John Condeelis, Thomas Harris, Thomas J. Ow, Michael B. Prystowsky, Geoffrey Childs, and Jeffrey E. Segall. “MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma.” Archives of Pathology & Laboratory Medicine, Special Section&; 8212;Head and Neck Squamous Cell Carcinoma, 139, no. 11 (November 2015): 1349–61. doi:10.5858/arpa.2014-0471-OA.
    • Vancouver/ICMJE:
      Jimenez L, Sharma VP, Condeelis J, Harris T, Ow TJ, Prystowsky MB, et al. MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma. Archives of Pathology & Laboratory Medicine [Internet]. 2015 Nov [cited 2020 Nov 23];139(11):1349–61. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=110709109