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Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling.

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  • Additional Information
    • Affiliation:
      Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge, Cambridge, U.K.
      Cambridge Oesophago-Gastric Centre, Addenbrooke's Hospital, Cambridge, U.K.
      INSERM U1016, Institut Cochin, Université Paris-Descartes, Paris, France
      Division of Gastroenterology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA
    • Subject Terms:
    • Subject Terms:
    • Abstract:
      Enteroendocrine cells (EECs) produce hormones such as glucagon-like peptide 1 and peptide YY that regulate food absorption, insulin secretion, and appetite. Based on the success of glucagon-like peptide 1-based therapies for type 2 diabetes and obesity, EECs are themselves the focus of drug discovery programs to enhance gut hormone secretion. The aim of this study was to identify the transcriptome and peptidome of human EECs and to provide a cross-species comparison between humans and mice. By RNA sequencing of human EECs purified by flow cytometry after cell fixation and staining, we present a first transcriptomic analysis of human EEC populations and demonstrate a strong correlation with murine counterparts. RNA sequencing was deep enough to enable identification of low-abundance transcripts such as G-protein-coupled receptors and ion channels, revealing expression in human EECs of G-protein-coupled receptors previously found to play roles in postprandial nutrient detection. With liquid chromatography-tandem mass spectrometry, we profiled the gradients of peptide hormones along the human and mouse gut, including their sequences and posttranslational modifications. The transcriptomic and peptidomic profiles of human and mouse EECs and cross-species comparison will be valuable tools for drug discovery programs and for understanding human metabolism and the endocrine impacts of bariatric surgery.
    • Journal Subset:
      Biomedical; Peer Reviewed; USA
    • ISSN:
      0012-1797
    • MEDLINE Info:
      PMID: NLM30733330 NLM UID: 0372763
    • Grant Information:
      MC_UU_12012/3/MRC_/Medical Research Council/United Kingdom; MC_UU_12012/5/MRC_/Medical Research Council/United Kingdom; /WT_/Wellcome Trust/United Kingdom; MC_UU_00014/3/MRC_/Medical Research Council/United Kingdom; MR/M009041/1/MRC_/Medical Research Council/United Kingdom; R01 DK110614/DK/NIDDK NIH HHS/United States; MR/M024873/1/MRC_/Medical Research Council/United Kingdom; R01 DK100223/DK/NIDDK NIH HHS/United States; 106262/WT_/Wellcome Trust/United Kingdom; MC_UU_00014/5/MRC_/Medical Research Council/United Kingdom
    • Publication Date:
      20190810
    • Publication Date:
      20200503
    • DOI:
      10.2337/db18-0883
    • Accession Number:
      136016674
  • Citations
    • ABNT:
      LARRAUFIE, P. et al. Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling. Diabetes, [s. l.], v. 68, n. 5, p. 1062–1072, 2019. DOI 10.2337/db18-0883. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=136016674. Acesso em: 25 set. 2020.
    • AMA:
      Larraufie P, Kay RG, Galvin SG, et al. Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling. Diabetes. 2019;68(5):1062-1072. doi:10.2337/db18-0883
    • APA:
      Larraufie, P., Kay, R. G., Galvin, S. G., Miedzybrodzka, E. L., Glass, L. L., Ma, M. K. L., Lam, B., Yeo, G. S. H., Chiarugi, D., Reimann, F., Gribble, F. M., Roberts, G. P., Richards, P., Hardwick, R. H., Scharfmann, R., Leiter, A., & Li, H. J. (2019). Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling. Diabetes, 68(5), 1062–1072. https://doi.org/10.2337/db18-0883
    • Chicago/Turabian: Author-Date:
      Larraufie, Pierre, Richard G. Kay, Sam G. Galvin, Emily L. Miedzybrodzka, Leslie L. Glass, Marcella K. L. Ma, Brian Lam, et al. 2019. “Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling.” Diabetes 68 (5): 1062–72. doi:10.2337/db18-0883.
    • Harvard:
      Larraufie, P. et al. (2019) ‘Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling’, Diabetes, 68(5), pp. 1062–1072. doi: 10.2337/db18-0883.
    • Harvard: Australian:
      Larraufie, P, Kay, RG, Galvin, SG, Miedzybrodzka, EL, Glass, LL, Ma, MKL, Lam, B, Yeo, GSH, Chiarugi, D, Reimann, F, Gribble, FM, Roberts, GP, Richards, P, Hardwick, RH, Scharfmann, R, Leiter, A & Li, HJ 2019, ‘Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling’, Diabetes, vol. 68, no. 5, pp. 1062–1072, viewed 25 September 2020, .
    • MLA:
      Larraufie, Pierre, et al. “Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling.” Diabetes, vol. 68, no. 5, May 2019, pp. 1062–1072. EBSCOhost, doi:10.2337/db18-0883.
    • Chicago/Turabian: Humanities:
      Larraufie, Pierre, Richard G. Kay, Sam G. Galvin, Emily L. Miedzybrodzka, Leslie L. Glass, Marcella K. L. Ma, Brian Lam, et al. “Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling.” Diabetes 68, no. 5 (May 2019): 1062–72. doi:10.2337/db18-0883.
    • Vancouver/ICMJE:
      Larraufie P, Kay RG, Galvin SG, Miedzybrodzka EL, Glass LL, Ma MKL, et al. Comparison of Human and Murine Enteroendocrine Cells by Transcriptomic and Peptidomic Profiling. Diabetes [Internet]. 2019 May [cited 2020 Sep 25];68(5):1062–72. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=rzh&AN=136016674